Clearing Agents, Mounting Agents, Dehydrating Agents, Embedding Materials Clearing Agents After dehydration the specimen is made more transparent by replacing the alcohol with a liquid of high refraction. Gradual transfer from alcohol to clearing agent is accomplished either by passing through an intermediate bath consisting of a mixture of alcohol and clearing agent, or by introducing the clearing agent under the alcohol and allowing slow diffusion to occur. When methyl benzoate is used, no intermediate bath is necessary. When dioxane or tetrahydrofuran is the dehydrating agent, no clearing agent at all is required. The following clearing agent and intermediate clearing mixtures are available:   Back to Top Mounting Agents Back to Top Dehydrating Agents Back to Top Embedding Materials Back to Top