Microbiology Stains
Microbiology stains are used in histology for the demonstration of bacteria and other microorganisms of interest in the related field.
Microbiology stains include a variety of stains and reagents. Some of the stains used are: Ziehl-Neelsen Carbol Fuchsin Solution, Methylene Blue Stock or Working Solution, Kinyoun's Carbol Fuchsin Solution, Auramine-Rhodamine, Carbol New Fuchsin Staining Solution, and Modified Van Gieson Stain. Stains are used for the demonstration of acid fast bacteria such as Nocardia and Lepra Bacilli.
For Gram+/Gram - bacteria , necrotic tissue and Erythrocytes the stains used are: Crystal Violet, 1% Aqueous, Basic Fuchsin Stock Solution, and 0.25%. Gram's Iodine Solution. For the demonstration of amoebae, the most popular stain used is Harris Hematoxylin without Mercury. Giemsa Stock is employed for the demonstration of H. Pylori and Mast Cells. Others stains used in microbiology for the diagnosis of H. Pylori are: Alcian Yellow, Toluidine Blue, 1% Aqueous Coleman's Fuelgen Solution, and Mayers Hematoxylin.
Chapman's modified Warthin Starry is a method use in Microbiology for the demonstration of Spirochetes, Donovan Bodies, and general bacteria. Grocott's Method (GMS) for Fungi uses Silver Nitrate 5% and 0.2% Aqueous Light Green Solution. In conjunction with the Microbiology Stain, some different reagents are used such as Gold Chloride 0.2%, Acetic Acid 3%, Chromic Acid 4%, and Sodium Thiosulfate 2% and 5%.